The genus Chamaesyce consists of approximately 250 species with a cosmopolitan distribution. The genus is best represented in the New World (ca. 80% of the species) and exhibits the highest amount of species diversity in Mexico. There are 22 species of Chamaesyce in Hawai`i. Fifteen species are endemic to the archipelago and seven are thought have been introduced and have become naturalized. The Hawaiian endemics are composed of semi-woody to arborescent taxa that are reputably derived from a single ancestor of the Pacific C. atoto complex. Cladistic analyses of nucleotide sequence data from the nuclear ITS and 5S-NTS loci were used to examine relationships among and between native and introduced species. Based on the species included in this study: (1) the Hawaiian taxa form two distinct clades, one containing arborescent taxa and the other containing semi-woody, non-arborescent taxa; (2) the herbaceous C. prostrata, generally considered naturalized in Hawai`i, occurs within the clade of non-arborescent Hawaiian taxa. This suggests that the Hawaiian species of Chamaesyce may not be monophyletic and that C. prostrata shares a common ancestor with the non-arborescent species. This research is supported by the Lewis B. and Dorothy Cullman Foundation.

Key words: 5S-NTS, Chamaesyce, Hawai`i, ITS