The Rapateaceae (17 genera, ca. 90 species) is largely restricted to the tepuis and sandplains of the ancient Guayana Shield in South America, with monotypic Maschalocephalus restricted to a region of West Africa that abutted the Guayana Shield prior to the opening of the Atlantic. Members of this family have undergone extensive radiation in flower form, leaf shape, leaf arrangement, and habitat. To provide a basis for analyzing patterns of geographical and morphological evolution in this group, we produced a molecular phylogeny based on a cladistic analysis of cpDNA sequence variation in the chloroplast-encoded ndhF gene, using several members of the Bromeliaceae as outgroups. Our strict consensus tree indicates that the lowland subfamily Rapateoideae is paraphyletic and includes the largely montane subfamily Saxofridericioideae as a monophyletic subclade; the tribe Saxofridericeae of the latter is paraphyletic, while tribe Schoenocephaliae is monophyletic. Spathanthus is sister to all other genera of Rapateaceae, with the tribes Rapateae and Monotremae representing successively divergent lineages within Rapateoideae. Saxofridericia/Stegolepis/ Epidryos/Marahuacaea, which have yellow, cup-shaped flowers and bee pollination, are sister to the tribe Schoenocephalieae, which have reddish, tubular flowers, the only nectaries in the family, and hummingbird pollination. Branch lengths are consistent with the operation of a molecular clock; Maschalocephalus is only slightly divergent from other Monotremae, suggesting that it is the product of a relatively recent, long-distance dispersal event, NOT continental drift. The distributions of present-day rapateads are consistent with the origin of the family in lowland inundated forests, followed by (i) colonization of lowland Amazonian savannas, (ii) invasion, frequent speciation, and evolution of hummingbird pollination in highland habitats atop tepuis (perhaps following uplift of the latter in association with the opening of the Atlantic), and (iii) reinvasions of lowland habitats by members of Saxofridericioideae and Schoenocephaliae.

Key words: Guayana Shield, hummingbird pollination, Rapateaceae, tepuis