SMEDMARK, JENNY AND TORSTEN ERIKSSON.* Department of Botany, Stockholm University, S-106 91 Stockholm, Sweden. - Geum and relatives (Rosaceae, Rosoideae), probable cases of bi-directional concerted evolution following allopolyploid speciation -- an interpretation based on nuclear ribosomal ITS and preliminary morpholological data.
The evolution of fruit characteristics in the Geum clade has
inspired previous researchers to come up with several genera. Some are
dispersed by wind, having plumose styles, and others by animals, for
example with the aid of hooked styles. A preliminary morphological
data set based mainly on previously used characters resulted in a
reasonable but very weakly supported tree when analysed
phylogenetically. Wind dispersed species came out basal which previous
analyses of rbcL (Morgan et al., 1994) and ITS (Eriksson et al., in
press) had indicated. We selected 19 species (covering Fallugia,
Geum in strict sense, Waldsteinia, Coluria, Acomastylis,
Erythrocoma, Sieversia, Novosieversia, and Oncostylus,
along with the outgroups Filipendula, Rosa, Rubus, and
Sanguisorba) and obtained sequences of the nuclear ribosomal
ITS region. The aligned data (670 sequence characters plus 17
informative gaps scored for presence or absence) were analysed using
parsimony in PAUP. There is some well supported resolution in the
strict consensus of the 39 shortest trees. For instance,
Fallugia and Sieversia are sister groups to rest of the
species. The monophyly of this less inclusive group is well supported
but the resolution is low within it, except for four species pairs.
Unexpectedly, the species differ markedly in morphology within the
pairs, and interpretations of fruit evolution seems unrealistic.
Examination of chromosome numbers and ploidy levels reveal possible
patterns of allopolyploid speciation with subsequent elimination of
one of the parental species ITS copies through concerted evolution. To
a large extent these conclusions support hypotheses formulated by
Gajewski (1957) based on cytogenetic studies. It is clear that the
ITS region may not be a suitable marker for the phylogeny within this
group. Further studies taking into account sequences of other DNA data
are in progress.
Key words: allopolyploidy, concerted evolution, fruit evolution, Geum, ITS, phylogeny