The family Staphyleaceae is a small dicotyledonous family of trees and shrubs traditionally thought to consist of five genera: Staphylea, Euscaphis, Turpinia (subfamily Staphyleoideae), Huertea, and Tapiscia (subfamily Tapiscioideae), totaling 50-60 species. Distributed across both the old and new world, the family is distinguished by having decussate, pinnate, serrate, stipulate leaves, small pentamerous flowers presented in paniculiform cymes, and fruits that are berries, inflated capsules or follicles. The relationship of Staphyleaceae to other families is controversial, and treatments have generally relegated the family to either the Cunoniales or Sapindales. Recent analysis of rbcL sequence data from representatives of each of the five genera supports a novel placement of the core Staphyleaceae (Staphylea, Turpinia and Euscaphis) within the Geraniales. The data does not, however, support monophyly of the family, suggesting a different ordinal placement for Huertea and Tapiscia. Sequences from the internal transcribed spacer region (ITS) of the 18S-26S nuclear ribosomal DNA were used to resolve species relationships within the family. Parsimony analysis of the ITS data set revealed relationships quite different form those supported by morphology. This analysis suggests that traditional generic groupings are polyphyletic and a re-evaluation of the delimitation of the genera is necessary.

Key words: Euscaphis, ITS, rbcL, Staphylea, Staphyleaceae, Turpinia