The relationship of the geophytic genus Peniocereus (Berger) Br. &. R. to other groups of cacti has been controversial. Previous authors have allied it with members of the Tribe Hylocereeae or to members of the Tribe Echinocereeae, citing morphological similarities with the Wilcoxia group of Echinocereus. Additonally, recent treatments of the Cactaceae have subsumed the genus Nyctocereus (Berger) Br. & R. within Peniocereus. Our present studies of DNA sequences of the plastid rpl16 intron have shown Peniocereus to be more closely allied with the Pachycereinae subtribe of Tribe Pachycereeae. Furthermore, the epiphytic species Deamia testudo, previously allied to Selenicereus (Tribe Hylocereeae), was also found to be part of this clade. The value of phylogenetic analyses of plastid rpl16 intron sequences to establish a revised classification for the Peniocereus-Nyctocereus clade is examined, particularly with respect to morphological diversification in Mexican Pachycereeae. With the inclusion of Deamia testudo in the Tribe Pachycereeae, this new evidence provides an example of another independent evolution of epiphytism in the Cactaceae, in addition to the well-known examples in tribes Rhipsalideae and Hylocereeae.

Key words: Cactaceae, classification, molecular systematics, Nyctocereus , Peniocereus, phylogeny