LOPEZ, NORMA I.*, SUZETTE COLON, WILLIAM CARROMERO, JAMES D. ACKERMAN, AND RAYMOND L. TREMBLAY. Department of Biology, University of Puerto Rico, PO Box 23360 San Juan, PR 00931-3360. - Genetic and morphological differentiation among Lepanthes caritensis, L. sanguinea, and L. eltoroensis (Orchidaceae).
Recently, Lepanthes caritensis was described as a new species
endemic to Puerto Rico. However, some believe this species to be
morphologically similar to L. sanguinea, also endemic to Puerto
Rico. In order to determine the taxonomic status for these species,
we analyzed genetic and morphological data. Starch gel
electrophoresis was used to estimate genetic divergence among these
species. L. eltoroensis was used as an out-group for all
analysis. Nine genetically discrete characteristics were found among
the species. Allele frequencies were significantly different among
species based on a chi-square. The UPGMA analysis, based on genetic
distance, showed a 33.8% differentiation between L. caritensis
and L. sanguinea. Morphological data consisted of nineteen
parameters used in a multivariate analysis. Only twelve variables
were considered for the final model. It differentiated the data in
three main groups, one for each species. However, there was little
separation between L. caritensis and L. sanguinea.
Genetic and morphological data showed differentiation between L.
caritensis and L. sanguinea. These results suggest that
L. caritensis and L. sanguinea are two species.
Key words: allozyme, genetic differentiation, Lepanthes, morphological differentiation, Orchidaceae