NIETO FELINER, GONZALO1*, JOSEP A. ROSSELLO PICORNELL2, AND JAVIER FUERTES AGUILAR1. 1Real Jardín Botánico, CSIC, Plaza de Murillo 2, 28014 Madrid, Spain, 2Dept. Biología Vegetal, Universidad de Valencia, Dr. Moliner 50, 46100 Burjassot, Valencia, Spain. - Extensive reticulate evolution in Armeria (Plumbaginaceae) based on nrDNA internal transcribed spacers.
The genus Armeria includes ca. 110 species distributed
primarily in the Northern hemisphere with its maximum diversity
concentrated in the western Mediterranean. Reticulation has been
proposed as a significant cause for such diversity based on various
evidence. To test this hypothesis with molecular data, a parsimony
analysis of 55 ITS sequences from 34 taxa was carried out. Results
yielded 30 most parsimonious trees differing only in terminal and
subterminal clades. Topology is otherwise consistent in recognizing 5
major well supported clades. Several facts suggest the interpretation
that shared nucleotide positions supporting the major clades are due
not to common ancestry but to extensive gene flow: 1) different
accessions (13) from the same subspecies (A. villosa subsp.
longiaristata) appear in three of the five major clades; 2)
each of the five major clades contains accessions of at least one of
the six subspecies recognized within A. villosa; 3) four out of
the five major clades are geographically restricted to clearly
delimited areas within southern Spain so that the composition of those
clades is congruent not with the systematic placement of the sample
but with its geographical origin. Further, this interpretation is
consistent with morphological patterns (viz. sympatric distinct taxa
sharing sets of morphological traits) as well as with previous
evidence revealing low internal reproductive barriers. Low homoplasy
levels (C.I.=0.85 excluding uniformative characters) indicate that the
trees fit very accurately the ITS data. On the other hand, high
consistency index is quite unexpected given the fact that
reticulations may introduce considerable distortion in cladograms. We
argue that active concerted evolution, documented using artificial
F1 hybrids and backcrosses, coupled with the low internal
barriers and with the extensive gene flow scenario can explain the low
homoplasy levels.
Key words: Armeria, hybrids, ITS, Plumbaginaceae, reticulate evolution