New World members of Acacia subgenus Acacia total approximately 60 species and are divisible into six groups of species that include the ant acacias and the A. macracantha, A farnesiana, A. rigidula, A. constricta, and A . acuifera species group. While the center of diversity of the subgenus is in Mexico, a cpDNA restriction site study found members of the West Indian A. acuifera species group to be ancestral within the subgenus. Moreover, most members of the A. acuifera species group are narrowly endemic on serpentine or karstic soils in Cuba and the Dominican Republic. These areas, and Caribbean coastal dry forests in general, have been cited as areas that may have served as refugia for an early Tertiary boreotropical flora. The center of diversity of the A. acuifera species group corresponds to the oldest landform in the Caribbean viz., eastern Cuba and western Hispaniola. Principal components analysis of morphological characters of members of the A. acuifera species groups revealed all taxa to be phenetically distinct. This morphological differentiation of the species also attests to their constituting an ancient radiation in these serpentine areas. Application of a modified island biogeography model to this radiation combined with what is known of the geological history of the Caribbean implicates both vicariance and dispersal in the evolution of these species.

Key words: Acacia, biogeography, cpDNA, Fabaceae, restriction site mapping, serpentine endemism