The phylogeny of two closely related genera, Cimicifuga and Actaea, was studied using sequences of the internal transcribed spacers of nuclear ribosomal DNA. Trollius and Beesia were chosen as the outgroups for the parsimony analysis. In the strongly supported monophyletic group containing the species of Cimicifuga and Actaea, Actaea species are nested within Cimicifuga and are most closely related to C. racemosa. Morphologically, C. racemosa has nearly sessile stigma and firm-walled follicle which are similar to the fruit morphology of Actaea. Therefore, the genus Actaea is most likely to have originated within the genus Cimicifuga with an ancestor that was closely related to C. racemosa. Biogeographically, the genus Actaea has a disjunct distribution in North America and Eurasia while C. racemosa, the single species in sect. Macrotrys of Cimicifuga, occurs in North America. The distribution pattern appears to suggest that Actaea was originated from North America. This study also indicates that the genus Beesia is a close relative of Cimicifuga and Actaea.

Key words: Actaea, Cimicifuga, ITS, phylogeny, Ranunculaceae