HORNER, HARRY T* AND NELS R LERSTEN. Department of Botany and Bessey Micrsocopy Facility, Iowa State University, Ames, IA 50011-1020. - A survey of types and distrbution of calcium oxalate crystals in leaves of Prunus sensu lato (Rosaceae).
There are only 11 published reports of crystals in the large family
Rosaceae. Except for an undocumented mention of raphides in
Quillaja, only druses and prismatics are known. There are only
three brief reports for Prunus s.l. (150-200 species; Subfamily
Prunoideae). We found previously unreported large adaxial,
sub-epidermal, crystal idioblasts with prismatics in leaves of
Prunus virginiana (choke cherry), which prompted us to survey
86 species of all subgenera and sections of Prunus s.l., as
well as two species of Quillaja. Small leaf samples taken
mostly from herbarium specimens were re-hydrated, partially cleared in
full-strength commercial bleach to preserve the crystals, rinsed
thoroughly, dehydrated, and permanently mounted. Samples viewed
between crossed polarizers using light microscopy showed crystal types
and sizes, location, and frequency. Dried or liquid-preserved leaf
samples of selected species were either broken in cross section or
peeled paradermally to expose the mesophyll and veins for scanning
electron microscopy observations. Druses (spherical aggregates of
crystals) are most common by far, typically in bundle sheath cells,
but in some species also in scattered mesophyll cells. Prismatics are
less common, usually among druses, but occuring in large sub-epidermal
idioblasts in a few species, of which P. virginiana and P.
padus are the best examples. P. serotina has myriad small
prismatics almost exclusively. This as yet incomplete survey, a
rarely done type of crystal study, indicates that crystals are
potentially useful taxonomic characters at sub-generic levels in
Prunus. The survey is also a useful frame of reference for
more intensive microscopic study on Prunus crystals, which is
in progress.
Key words: calcium oxalate, crystal distribution, leaves, Prunus