OWENS, SHIRLEY A. Laser Scanning Microscope Lab, Department of Crop and Soil Sciences, Michigan State University, East Lansing, MI, 48824-1325. - Secondary pulvini in the leaves of Cercis canadensis L. (Fabaceae).
Cercis canadensis (Fabaceae, Caesalpinnoideae) has a simple,
palmately veined leaf with a large upper pulvinus terminating in a
cushion of pulvinus tissue that extends into the lamina. Controversy
over how this leaf type, unique within the Fabaceae to the tribe
Cercideae, was derived has long been debated. The derivation of this
leaf is thought to be from either the fusion of leaflets of a
palmately or pinnately compound leaf or the reduction of a compound
leaf to a single terminal leaflet. Microscopic observations of the
upper pulvinus in C. canadensis showed three cylinders of
vascular tissue surrounded by pulvinus tissue. The central cylinder
becomes the midrib and is formed from two adaxial and a central
abaxial bundle at the proximal end of the upper pulvinus. Lateral
vascular cylinders separate into three primary veins within the upper
pulvinus. Partially fused secondary pulvini at the base of each
primary vein form the pulvinus cushion. The pulvini and associated
vascular tissue are homologous to those reported for leaflets. A
mucro at the distal end of the midrib that abscises shortly after the
unfolding of the lamina indicates that the leaflets originally formed
a pinnately compound structure. This was expected as pinnately
compound leaves are characteristic for the subfamily Caesalpinnoideae.
Measurements of movement (over 12-hours) of the midrib with respect
to the petiole and the lamina basal lobes with respect to the midrib
were done on 24 leaves growing on six branches of two trees. All of
these leaves were harvested for microscopic observation. Changes in
the leaf movements studied can be attributed to the expansion and
contractions of the partially fused secondary pulvini. Additional
evidence that fusion of leaflets from a pinnately compound leaf gave
rise to the simple leaf in Cercis is presented.
Key words: Cercis canadensis, pulvinus cushion, secondary pulvini