Plant hybridization can result in hybrids having similar characteristics of either pure parental or combinations of both. Characters such as anatomy and morphology, leaf chemistry, susceptibility to herbivores etc., may be quite variable relative to the pure parentals. However one aspect of hybridization less studied is physiological response to herbivores. This trait could have important consequences for fitness if the hybrids responded to herbivory by allocating carbon and water differently than the pure parentals. A natural hybrid zone of Populus along the Weber River in northern Utah provides a unique opportunity to examine photosynthetic rates and water relations in response to herbivores of different feeding types. We examined water potential, photosynthesis and water use efficiency of both naturally occurring and common garden trees of known genotype. These trees consisted of Populus fremontii, Populus angustifolia, their F1 hybrids and complex backcross hybrids. The measured variables were quantified from both naturally occurring and experimental manipulations of a galling aphid, a free feeding aphid and a free feeding beetle. Leaves with the herbivore, adjacent leaves without the herbivore and leaves on a herbivore free shoot were measured to examine how tree type and herbivore affected the physiological responses that leaves exhibited. The results indicate that both tree type and herbivore affect leaf level physiological processes. All three of these herbivores occur most frequently on hybrid classes than on the pure parentals and have different impacts on these trees. The galling aphid, as a mobilizing sink, had the greatest impact on both photosynthesis and water relations compared to responses to the other herbivores.

Key words: hybridization, photosynthesis, plant-herbivore interactions, Populus, water use efficiency