HERZ, MEGAN, RACHEL KOCH, JOHN GAVENCAK, AND RICHARD A. NIESENBAUM.* Biology, Department Muhlenberg College, Allentown, PA 18104-5586. - Does the proximity of developing selfed and outcrossed fruits influence selective abortion and inbreeding depression in Mirabilis jalapa?
When flowers of the self-compatible herb Mirabilis jalapa are
pollinated with either self or outcross pollen randomly over the
entire plant, there is no selective abortion of selfed fruits or
seeds, or any evidence of inbreeding depression. We tested the
hypothesis that when developing selfed and outcrossed fruits are in
close proximity to each other and in direct competition for resources,
there will be an increase in the abortion of selfed fruits and in the
expression of inbreeding depression. This was done by performing
clustered pollination treatments, such that all flowers on a branch
either received all self pollen, all outcross pollen from a variety
of donors, or flowers within a cluster were alternately pollinated
with self or outcross pollen. We predicted that differences in
rates of abortion, seed size, and/or seedling vigor would be greatest
between selfed and outcrossed treatments on mixed clusters.
Pollination treatment and cluster type had no influence on rates of
abortion or seed size. Germination and seedling performance were not
different for self and outcross treatments for unmixed clusters;
however, outcrossed seeds and seedlings significantly out performed
those from self pollinations from mixed clusters. This suggests
that when developing in close proximity and potentially competing for
resources, outcrossed seeds have an advantage over selfed seeds,
but this advantage is not observable until later in the plant’s
life-cycle when seeds are planted in the field.
Key words: inbreeding depression, Mirabilis jalapa, resources, selective abortion, self pollination