Pericallis includes 14 species endemic to the archipelagos of Azores, Canary Islands, and Madeira. Species in the genus occur in all five ecological zones except the high elevation desert and they exhibit considerable variation in growth form and floral biology. We have sequenced the internal transcribed spacer (ITS) region of the nuclear ribosomal DNA for all species of Pericallis and its putative continental relatives to examine the origin and evolution of the genus. The ITS trees indicate a close relationship between Pericallis and the African genera Dendrosenecio and Cineraria and also to the New World genera Pseudogynoxys and Dorobaea. This represents the first phylogenetic evidence linking a member of the Macaronesian Flora with taxa from the New World. Patterns of evolution in Pericallis were also assessed within Macaronesia using the ITS trees. The major trend is that species from the same ecological zones on different islands form monophyletic groups. This indicates that inter-island colonization between similar ecological zones on different islands is the major avenue of evolution, a pattern which we have observed in several other Macaronesia endemics (i.e., Argyranthemum, Crambe, Silene).

Key words: Asteraceae, ITS phylogeny, Macaronesian flora, Pericallis, Senecioneae