|
 |
|
|
Assistant Professor of Zoology
Phone: (405)325-9324
Fax: (405)325-6202RM/Lab:RH407/RH205
Wang Lab Web Page
Current Research Interests and Subject Areas Available for Graduate Research
Molecular Genetics of Zebrafish Circadian Rhythmicity
Most organisms display daily changes at molecular, cellular, physiological, and behavioral levels, which is known as circadian rhythms. Circadian clocks control fundamental processes such as the human sleep-wake cycle. Many human sleep disorders are thought to result from circadian defects. A full molecular and genetic understanding of circadian rhythmicity could help cure human dysrhythmias.
We use the zebrafish (Danio rerio) as our model because it allows for a unique approach that integrates molecular, developmental, genetic and genomic techniques. We isolate zebrafish circadian clock genes and circadian clock-controlled genes and profile oscillating patterns of these genes in early embryos and organs such as the brain and the eyes. To elucidate the functions of these genes, we overexpress these genes and "knock them down" in living zebrafish embryos. We generate transgenic zebrafish using putative promotes of the selected circadian clock genes ligated with jellyfish green fluorescent protein (GFP). Those GFP-oscillating zebrafish will be mutagenized and screened for circadian mutants.
To learn more about this research, visit the Wang lab web page.
M.S., Chinese Academy of Sciences
B.S., Anhui University
Member of the Oklahoma Center for Neuroscience
Selected publications:
A. E. Tucker, I. I. Salles, D. E. Voth, W. Ortiz-Leduc, H. Wang, I. Dozmorov, M. Centola and J. D. Ballard. 2003. Decreased glycogen synthase kinase 3-beta levels and related physiological changes in Bacillus anthracis lethal toxin-treated macrophages. Cellular Microbiology 5:523-532.
R. M. Young , S. Marty , Y. Nakano, H. Wang, D. Yamamoto, S. Lin, and M. L. Allende. 2002. Zebrafish yolk-specific not really started (nrs) gene is a vertebrate homolog of the Drosophila spinster gene and is essential for embryogenesis. Developmental Dynamics 223:298-305.
H. Wang, Q. Long, S. D. Marty, S. Sassa, and S. Lin. 1998. A zebrafish model for hepatoerythropoietic porphyria. Nature Genetics 20:239-243.
Q. Long, A. Meng, H. Wang, J. R. Jessen, M. J. Farrell, and S. Lin. 1997. GATA-1 _expression pattern can be recapitulated in living transgenic zebrafish using GFP reporter gene. Development 124:4105-4111.
- H. Wang, E. D. McArthur, S. C. Sanderson, J. H. Graham, and D. C. Freeman. 1997. Narrow hybrid zone between two subspecies of big sagebrush (Artemisia tridentata: Asteraceae). IV. Reciprocal transplant experiments. Evolution 51:95-102.
|
|
|||
|
|||
|
|
|
|
|
|
|
