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Mass Spectrometry

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Mass Spectrometry

The OU Mass Spectrometry (MS) Core Facility operates, maintains, and services numerous mass spectrometers and ultra-high-pressure liquid chromatographs (UPLC). The facility provides a broad range of services and is located in the Stephenson Life Sciences Research Center at 101 Stephenson Parkway, Room 1301. The facility director, Steven Foster, has over 21 years of MS experience and is available to help answer any questions you may have. His contact information is below.

Services

Our services include for-fee sample analysis, user run open access instrumentation (after proper training), teaching and education, consulting, and collaborative research projects. In the past we have specialized in small molecules and metabolomics, but we are progressing into the world of large molecules and proteomics. No matter what your mass spectrometry and HPLC needs are we will try to assist you or direct you to someone who can.

Instruments

UPLC / Q-TOF MS (2016)
Consists of an Agilent 6545 high-mass-resolution Q-TOF mass spectrometer and an Agilent 1290 UPLC (1200 bar). The Mass Hunter data-control system includes qualitative and quantitative analysis as well as advanced metabolomics software, Mass Profiler Pro. The ultra-high mass resolution and UPLC allows for the separation of more than 1,000 compounds in a single LC/MS run. This system includes Agilent Rapid Fire 365 high-throughput automated sample prep robotics.


UPLC / Ion Mobility / Q-TOF MS (2017)
Comprised of a Waters SYNAPT G2-Si Q-TOF/MS and a Waters M-Class chromatography system that can be set up as micro-flow UPLC or nano-LC. With the new technology of high-efficiency T-Wave ion mobility, we can obtain an additional dimension of separation based on molecular size and shape. The wide range of benefits feature isomer separation, elimination of interferences, generation of cleaner spectra, and the ability to better confirm compound ID.


Orbitrap Ultra High-Resolution MS (2019)
Thermo-Scientific Orbitrap Fusion Lumos Tribrid MS with nano-LC excels at the most challenging applications, including low level PTM analysis, multiplexed relative quantitation using isobaric tags, intact Top-down protein characterization, as well as MSn analysis of molecules. It has the highest capabilities of any mass spectrometer in Oklahoma.


ICP MS with Laser Ablation (2018)
PerkinElmer NexION2000 Inductively Coupled Plasma (ICP)-MS with Single Cell analysis capability, Elemental Scientific Inc (ESI) - New Wave Research 213 nm Laser Ablation system and ESI prepFAST IC automation with integrated autosampler and LC capability. This system is ideal for analysis of metals in soil, rock surface, bird feathers, bones, plants, and single cell biomedical applications.


(2) GC / quadrupole MS (1998 & 2018)
Agilent 6890N gas chromatograph (GC) with an Agilent 5973 Mass Selective Detector (MSD) and electron impact (EI) ion source. This is specific for volatile compounds and gases from 20-600 mass, notably benzenes, alcohols and aromatics. It is often used in toxicology, pesticide analysis, and the oil & gas industry.

Location

Contact

Steven Foster
Phone: 405-325-4749
Email: fosters@ou.edu

Address

Stephenson Life Sciences Research Center
101 Stephenson Parkway
Rm 1301
Norman, OK 73019