MALEATE BUFFER, 0.05 M

Components:

Formula for 0.2 M maleate stock solution:

for 100 ml

for 200 ml

for 500 ml

Maleic acid

2.32 g

4.64 g

11.6 g

1 N NaOH

20 ml

40 ml

100 ml

Distilled H2O

to 100 ml

to 200 ml

to 500 ml

Formula for 0.05 M maleate, pH 5.15:

for 100 ml

for 200 ml

0.2 M maleate stock

25 ml

50 ml

0.2 N NaOH

4.2

8.4

Distilled H2O

to 100 ml

to 200 ml

Titrate buffer with 0.2 N NaOH until pH 5.15 is reached. (This is used as the rinse buffer for animal tissue and is used before and after en bloc staining with uranyl magnesium acetate)

Formula for 0.05 M maleate, pH 6.0:

for 100 ml

for 200 ml

0.2 M maleate

25 ml

50 ml

0.2 N NaOH

to pH 6.0

to pH 6.0

Distilled H2O

to 100 ml

to 200 ml

Titrate with NaOH until pH 6.0 is reached. (This is the staining buffer used to make the uranyl magnesium acetate solution used for en bloc staining)

Reference: Temple, 1929. J.A.C.S. 51: 1754.