Principles and Techniques of Transmission
Electron Microscopy
BIOL/MBIO/PBIO 5364 –
Tentative Syllabus – Fall 2013
Samuel
Roberts Noble Microscopy Laboratory Web site:
http://www.microscopy.ou.edu/
SRNEML
Laboratory Phone: 325-4391
D2L Class Website:
Instructors:
Scott Russell, GLC Professor, and Director Office: 210 NML e-mail:
srussell@ou.edu
Preston Larson, Research Scientist Office:
205E NML e-mail: plarson@ou.edu
Greg Strout, Electron Microscopist Office:
214 NML e-mail: gstrout@ou.edu
Lecture:
MWF 8:30-9:20 am NML room 212
Laboratory:
TBD, TEM: NML room 203
Textbooks for Reference (choose one,
depending on research interests):
Material
Sciences: D. B. Williams and C. B. Carter,
“Transmission Electron Microscopy: A Textbook
for Material Scientists” (1996) Plenum Press, New York, NY. ISBN 0-306-45247-2
(Hardcover). - OR - D. B. Williams and C. B. Carter, “Transmission
Electron Microscopy, 2nd edition” (2009) Springer Verlag,
New York, NY. ISBN 978-0-387-76500-6
(Hardcover) 837 pp. $149, 978-0-387765020
(Softcover) $99 (also Kindle Edition) (Amazon
has new copes of these for $98.23 and $70.99, respectively, on 8/19/2012)
Biological
Sciences: J. J. Bozzola and
L. D. Russell, “Electron Microscopy:
Principles and Techniques for Biologists”, 2nd ed. (1999),
Jones & Bartlett. ISBN
0-7637-0192-0 (Hardcover), pp $160.95 retail. (also
Kindle Edition) (Amazon has new copies at retail as of 9/28/12, used are less)
Tentative Course Schedule (as of 10/18/13)
Week |
Date |
Lecture |
Week 1 |
Aug. 19 |
Course content,
requirements, grading, lab rules, lab tour, Intro to Electron Microscopy (SR) |
Aug. 21 |
Instrument, Gun,
and Optical Principles (PL) |
|
Aug. 23 |
Instrument, Gun, and
Optical Principles (PL) |
|
Week 2 |
Aug. 26 |
Electromagnetic
Lenses & Optics (SR) |
Aug. 28 |
Electromagnetic
Lenses & Optics (SR) |
|
Aug. 30 |
Instrument Training
Session #1 (GS) |
|
Week 3 |
Sep. 2 |
Labor Day |
Sep. 4 |
Instrument Training
Session #2 (GS) |
|
Sep. 6 |
Instrument Training
Session #3 (GS) |
|
Week 4 |
Sep. 9 |
Vacuum Systems
& Technology (PL) |
Sep. 11 |
Vacuum Systems
& Technology (PL) |
|
Sep. 13 |
Electron Sources
(PL) |
|
Week 5 |
Sep. 16 |
Materials Specimen
Preparation (PL) |
Sep. 18 |
Support Film, Replicas,
Shadow Casting (SR) |
|
Sep. 20 |
Life Sciences Prep
/ Ultramicrotomy (SR) |
|
Week 6 |
Sep. 23 |
Beam Specimen
Interactions (PL) |
Sep. 25 |
Exam 1 |
|
Sep. 27 |
Individual Class
Projects |
|
Week 7 |
Sep. 30 |
Energy Dispersive
Spectroscopy (PL) |
Oct. 2 |
Energy Dispersive
Spectroscopy (PL) |
|
Oct. 4 |
Freezing
Technologies/Freeze Fracture (SR) |
|
Week 8 |
Oct. 7 |
End Fracture/Cryoultramicrotomy (SR) |
Oct. 9 |
Image
Interpretation and Artifacts (SR) |
|
Oct. 11 |
Dallas Football
Weekend |
|
Week 9 |
Oct. 14 |
TEM Imaging Modes:
Imaging Principles, Resolution, Ray Paths (PL) |
Oct. 16 |
TEM Imaging Modes:
Diffraction, Bright Field, Dark Field, STEM, HAADF (PL) |
|
Oct. 18 |
High Voltage EM
(SR) |
|
Week 10 |
Oct. 21 |
Quantitative
Microscopic Analysis and Stereological Methods (SR) |
Oct. 23 |
Electron Tomography
(SR) |
|
Oct. 25 |
High Resolution TEM
(PL) |
|
Week 11 |
Oct. 28 |
Electron Energy
Loss Spectroscopy (PL) |
Oct. 30 |
Demo: Digital Imaging
(GS) |
|
Nov. 1 |
Exam 2 |
|
Week 12 |
Nov. 4 |
|
Nov. 6 |
||
Nov 8 |
OAS Fall Meeting @
Cameron University |
|
Weeks 13-16 |
Nov. 11-Dec 6 |
Poster Making / |
Fall
2013 Academic Calendar:
http://www.ou.edu/admissions/home/academic_calendar/fall_2013.html
Final Examination
Schedule and Rules:
http://www.ou.edu/content/enrollment/home/final_exams/fall_semester_final0.html
INSTRUCTIONAL GOALS OF THE COURSE
The goal of the
course, Transmission Electron Microscopy (TEM), is to develop within students appreciation and operational ability to conduct
independent research using a transmission electron microscope as well as an
understanding of the principles underlying and preparation of materials for
observation using TEM. This course
combines study of physical and optical principles underlying TEM, as well as
hands-on training, operational procedures, basic trouble-shooting and alignment
of the Zeiss 10 TEM.
As part of the course, each student will conduct a research project
using a transmission electron microscope and preparatory equipment in the NML
to prepare their own material. The
course will be graded by the student’s ability to take written examinations,
focus and stigmation of model specimens, demonstrate
practical and operational knowledge of the operation of the transmission
electron microscope through written and oral examinations including operation
and trouble-shooting, and presenting their research results in a poster and
class presentation. At the end of the
course, the student will have practical and applied knowledge of transmission
electron microscopy in the physical and biological sciences and be able to
prepare samples and operate a transmission electron microscope.
FINAL PROJECTS
Projects
are presented in lieu of a final examination.
All projects are due at the final:
10:30
AM on Thursday, December 12, 2013
Briefly,
the project involves:
STIGMATION MICROGRAPHS
Final stigmation/focus
series photographs are due on or before December 6th. Up to three sets of stigmation/focus
series images may be turned in and only the highest grade will count. The first set will be due on October 30th with the final
set due on or before December 6th.
The stigmation micrographs consist of:
EQUIPMENT CHECK-OUT
Zeiss 10: Checkouts begin on October 9th Check-outs may include both a written and
oral/hands-on component. The length of
an individual student’s checkout is variable depending on their knowledge of
the microscope, principles of electron microscopy and operational skill, but
plan on allotting around 4-6 hours.
Checkouts on both TEMs will consist of the following parts:
If a need exists for
you to use the JEOL 2000FX (e.g. higher
resolution), consult the instructor to discuss the possibility of training on
this machine. Training on the JEOL 2000FX
will be based on research needs, competence (partly based on the Zeiss 10 checkouts), time constraints, and the discretion
of NML personnel.
JEOL 2000FX: Checkouts will be conducted at the discretion
of NML personnel.
Other Equipment: Users must be checked
out to use expensive pieces of preparatory equipment. Contact NML personnel for training. To keep training consistent, non-NML personnel are not permitted to conduct separate
training. Supervised operation is
required until you are competent and checked out.
User’s List: A list of users of
NML equipment and the instruments they are qualified to use is maintained by
the lab. DO NOT USE any equipment on
which you have not been checked out.
Only NML personnel are to train individuals on the use of NML equipment.
Note: It is
recommended that you begin working on your project prior to completing your
checkout.
Online schedule. The sign-up sheet and
schedules for the Zeiss 10 and JEOL 2000 are located
online at: http://www.microscopy.ou.edu/schedule/index.cgi
You will be entered
into the system when you become knowledgeable and independent enough to use the
microscope without EM personnel present.
Consult NML personnel before scheduling equipment use.
GRADING
Grading in the course will be broken down as
follows:
Exam 1 20%
Exam 2 20%
Stigmation & Focus Series 5%
Checkouts 15%
Final Project/Poster
Presentation 40%
Total 100%
POLICY
All OU students are governed by the policies of
the University of Oklahoma that are currently in force. Policies are provided online at URL: http://catalog.ou.edu/current/Policies.htm
and include all associated links and references from that page. Particularly, these include the OU Student
Code (http://www.ou.edu/content/dam/studentlife/documents/AllCampusStudentCode.pdf)
and the Academic Misconduct Policy (http://integrity.ou.edu/),
to name the two most important references.
Any and all instances of Academic Misconduct threaten the quality of
students’ learning experiences and therefore are given special scrutiny by the
university.
All of these stated
policies are incorporated into this syllabus by this reference. It is ultimately the student’s responsibility
to be aware of current and changing policies over the course of their academic
career. All participants in the course
are bound by these policies.