Contact Steve Barlow Phone: (619) 594-4523
FAX: (619) 594-5676
email: sbarlow@sunstroke.sdsu.edu
SEE BELOW FOR ALTERNATIVE DATES/SITES
Routine processing of tissue for transmission electron microscopy in clinical and research applications is a time consuming process. This workshop focuses on the use of a laboratory microwave oven as a means to accelerate all aspects of tissue processing for TEM. The demonstrations, course handouts and "hands-on" experience will provide workshop participants with the tools necessary to rapidly assimilate a laboratory microwave into their protocols. Other areas covered in the workshop include microwave demonstrations of histological staining (periodic acid-methenamine silver stain of kidney basement membranes), immunostaining for TEM and digital image acquisition and printing. Each workshop is an intensive two and a half-day experience based on the following:
Giberson, R.T., Demaree, R.S., Jr. (1995) Microwave fixation: Understanding the variables to achieve rapid reproducible results. Micros. Res. & Tech (in press)
Giberson, R.T., Smith, R.L., Demaree, R.S. (1995) Three hour microwave tissue processing for transmission electron microscopy: From unfixed tissue to sections. Scanning 17, Suppl. V:26-27
Demaree, R.S., Jr., Giberson, R.T., Smith, R.L. (1995) Routine microwave polymerization of resins for transmission electron microscopy. Scanning 17, Suppl. V:25-26
"Hands-on" Microwave Workshop for TEM and Histology Calif. State University, Chico, June 15-17, 1995
COURSE OUTLINE, SAN DIEGO STATE UNIVERSITY
Jan 25, 1996 8:30-5:00 Lecture and microwave demonstration by R.
T. Giberson
Demonstration of 3 hour microwave tissue processing
Jan 26, 1996 8:00-5:00 Participants will work in groups of 5 and
process tissue using 3
hour protocol in laboratory microwaves
Jan 27, 1996 8:00-12:00 Microwave demonstrations of on-grid
immunostaining, histological
staining, and digital imaging
Registration Fee for the SAN DIEGO WORKSHOP (workshop limited to 20
participants)
Workshop ONLY (includes the following) $500.00
1. Group dinner at the end of the first day
2. Lunch the first two days
3. Course handouts and all materials used in the workshop
4. Certificates of Completion mailed to each participant
Workshop AND 3 DAYS LODGING $700
at local hotel for 3 nights starting the night before the
workshop
1. Group dinner at the end of the first day
2. Lunch the first two days
3. Course handouts and all materials used in the workshop
4. Certificates of Completion mailed to each participant
In the event a participant needs to cancel, written notification (fax is acceptable) is required. A $50.00 administrative fee will be charged for cancellations. 50% of the registration fee will be reimbursed for cancellations within 30 days of the workshop. Substitutions are acceptable.
University of Maryland Dept. of Zoology, College Park, MD JAN 3-5, 1996
Contact Tim Maugel- Phone (301) 405-6898
FAX: (301) 314-9358
email: maugel@zool.umd.edu
Yale University School of Medicine New Haven CT JAN 10-12, 1996
Contact Paul Webster Phone: (203) 785-3219
FAX: (203) 785-7226
email: paul_webster@quickmail.yale.edu
NIH/Rocky Mountain Labs, Hamilton, MT JULY 1-3, 1996
Contact Dave Dorward Phone:(406) 363-9266
FAX: (406) 363-9204
email: dave_dorward@nih.gov
Madison Area Technical College, Madison, WI AUG 7-9, 1996
Contact Michael Kostrna Phone:(608) 246-6762
FAX: (608) 246-6880
email: msk5063@madison.tec.wi.us
Dr. Steve Barlow
EM Facility/Biology Dept.
San Diego State University
San Diego CA 92182-4614
phone: (619) 594-4523
fax: (619) 594-5676
email: sbarlow@sunstroke.sdsu.edu