3-D Microscopy of Living Cells

University of British Columbia, Vancouver, BC, Canada

July 27 - August 4, 1996

Covering everything from basic microscopy to the technical considerations that define the highest levels of performance of the confocal microscope, this course will include:

Quantitative confocal microscopy
Pixelation: The Nyquist Criterion
Lasers and laser tweezers
Objectives and aberrations
Scanning-systems
Wide field/deconvolution techniques
Detectors: operation and performance
Optimal pinhole size & photon efficiency
Dye design, characteristics and use
How to keep your cells alive
Two-photon excitation
Video-rate confocal imaging
Measuring ion concentrations
Display and measurement of 3D data
Digital hard copy and storage
Fluorescent & gold labeling of living cells
Backscattered light imaging.

Lecture demonstrations will be interspersed with hands-on laboratory exercises that will utilize all of the currently available commercial instruments for 3D microscopic imaging.

Students will work in groups of three throughout the discussion and laboratory sessions, and will complete a live-cell 3D study on a specimen of their choice.

At least seven, separate 3D microscopical workstations, attended by a technical staff of 15, will be available for student use. Overall, the teacher/student ratio will be more than 2:1.




                        International Academic Faculty:



        *       Jon Art                 University of Illinois

        *       Milton Charlton         University of Toronto

        *       Rachel Errington        Oxford University

        *       John Heuser             Washington University

        *       Jim Pawley              University of Wisconcon-Madison

        *       Wallace Marshall        U. of  California, San Francisco

        *       Ernst Stelzer           EMBL, Heidelberg

        *       Roger Tsien             University of California, San Diego

        *       Watt Webb               Cornell University

        *       Michael Weis            University of British Columbia

        *       Nick White              Oxford University



                        International Commercial Faculty:



        *       Ernst Keller            Carl Zeiss, NY

        *       Paul Millard            Molecular Probes, OR

        *       Sigrid Myrdal           Bristol-Myers Squibb, WA

        *       K. Sam Wells            Bio-Rad,,CA

Tuition

Tuition is $1,500 US. On receipt of the deposit, all students will receive preliminary assignments and a copy of the textbook, "Handbook of Biological Confocal Microscopy," (Plenum, 1995).

The tuition fee includes one ticket for the opening reception and the banquet, the textbook and all handouts. Accommodations and meals are not included in the tuition fee.

Applications

Applicants will complete a questionnaire to assess knowledge level and field of interest. Enrolment will be limited to 20 participants. Selection will be made on the basis of background and perceived need. Those without previous LM experience will be provided with basic texts to read before the course begins. Application packages may be obtained from




                Prof. James Pawley, Rm. 1235,

                1500 Johnson Dr., Madison, WI, USA 53706.

                Phone: 1-608-263-3147, Fax 1-608-265-5315,

                Email: jbpawley@facstaff.wisc.edu

Application deadlines

Applications forms requesting information on field of interest and level of experience must be received for screening by March 1, 1996.

Successful applicants will be notified by April 1, and a deposit of 50% must be received by April 15, 1996. In general, refunds of the deposit will not be possible.

Dates


        Applications must be received by        Mar. 1/96

        50% deposit due                         Apr. 15/96

        Registration 3:00 - 5:00 pm             Sat., July 27/96

        Last class will end with lunch          Sun., Aug. 4/96