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Kellye Cupp-Sutton

Kellye A. Cupp-Sutton

Kellye A. Cupp-Sutton

Postdoc Research Associate

Supervisor: Wu
Email:
 kellyecuppsutton@ou.edu

Education:
B.S.
, Chemistry, University of Central Oklahoma
Ph.D.
, Chemistry, University of Oklahoma


Kellye A. Cupp-Sutton is bioanalytical chemist specializing in mass spectrometry-based proteomics. She earned a Bachelor of Science in Chemistry at The University of Central Oklahoma in 2013 and subsequently earned a Ph.D. in Chemistry at The University of Oklahoma, Norman with Dr. Michael T. Ashby in 2018. Her graduate research focused on examination of the kinetics and mechanism of lactoperoxidase mediated oxidation of thiocyanate using stopped-flow UV-Vis spectroscopy which challenged a long standing mechanism. Upon graduation, she began postdoctoral research with Dr. Si Wu at The University of Oklahoma. Her research has focused on the development of quantitative mass spectrometry-based top-down proteomics methods for high-throughput functional proteomics. Primarily, she has developed a top-down thermal proteome profiling platform to study the effect of protein modification on protein thermal stability. In addition to her research, she has helped mentor twelve graduate and seven undergraduate students in diverse areas of research including single-cell proteomics, high throughput hydrogen-deuterium exchange mass spectrometry, and intact protein isobaric chemical tag labeling for complex samples resulting in authorship or co-authorship of thirteen research articles, reviews/perspective, and a book chapter in addition to presentations at national and international conferences.


Publications

  • Cupp-Sutton, K.A., Fang, M, Wu, S. (2022) Separation methods in single-cell proteomics: RPLC or CE? International Journal of Mass Spectrometry. (Young Scientist Feature, Submitted)
  • Guo, Y, Yu, D, Cupp-Sutton, K.A., Liu, X, Wu, S. (2022) Optimization of protein-level tandem mass tag (TMT) labeling conditions in complex samples with top-down proteomics. Analytica Chimica Acta. 1221: 340037.
  • Cupp-Sutton, K.A., Ashby, M. T. (2021) Reverse Ordered Sequential Mechanism for Lactoperoxidase with Inhibition by Hydrogen Peroxide. Antioxidants. 10(11):1646.
  • Huang, L., Fang, M., Cupp-Sutton, K.A., Wang, Z., Smith, K., and Wu, S. (2021) Spray-capillary based capillary electrophoresis mass spectrometry for metabolite analysis in single live cells. Analytical Chemistry. 2021 Mar 16;93(10):4479-4487.
  • Yu, D., Wang, Z., Kou, Q., Cupp-Sutton, K.A., Smith, K., Liu, X., and Wu, S. (2021) Quantitative top-down proteomics in complex samples using protein-level tandem mass tag (TMT) labeling. Journal of the American Society for Mass Spectrometry. 32(6): 1336-1344.
  • Cupp-Sutton, K.A., Wang, Z., Yu, D., and Wu, S. (2020) RPLC-RPLC-MS/MS for proteoform identification. Proteoform Identification. Methods and Protocols in Methods in Molecular Biology. 2500: 31-42.
  • Fang, M., Wang, Z., Cupp-Sutton, K.A., Welborn T., Smith, K., and Wu, S. (2020) High-throughput hydrogen deuterium exchange mass spectrometry (HDX-MS) coupled with subzero temperature ultrahigh pressure liquid chromatography (UPLC) separation for complex sample analysis. Analytica Chimica Acta. 1143: 65-72.
  • Wang, Z., Yu, D., Liu, X., Cupp-Sutton, K.A., Smith, K., and Wu, S. (2020) Development of an Online 2D Ultrahigh-Pressure Nano-LC System for High-pH and Low-pH Reversed Phase Separation in Top-Down Proteomics. Analytical Chemistry. 92(19):12774-12777.
  • Cupp-Sutton, K.A., and Wu, S. (2020) High-throughput Quantitative Top-Down Proteomics. Molecular Omics. 16(2): 91-99. (Invited Review)
  • Huang, L., Wang, Z., Cupp-Sutton K.A., Smith, K., Wu, S. (2019) Spray-Capillary: An Electrospray Assisted Device for Quantitative Ultra-Low Volume Sample Handling. Analytical Chemistry.
  • Yu, D., Wang, Z., Kou, Q., Cupp-Sutton, K.A., Smith, K., Liu, X., and Wu, S. (2019) Deep Intact Proteoform Characterization in Human Cell Lysate using High-pH and Low-pH Reversed Phase Liquid Chromatography. JASMS.
  • Cupp-Sutton K. A., and Ashby M. T. (2016) Biological Chemistry of Hydrogen Selenide. Antioxidants (5) 42. (Invited Review)