T.E.M. SPECIMEN PREPARATION DATA SHEET:

Description of experiment, treatment of experimental materials:

 

 

VIALS:    
Fixation: ___________ in _________ buffer at ___ °C pH ___    
Rinse buffer: (___ X ___ min/each) at ___ °C pH ___ 1.    
  2.    
  3.    
  4.    
Fix: __ % OsO4 (buffered in ________) at ___ °C pH ___    
Rinse: (___ X ___ min/each in ______) at ___ °C pH ___      
       
Dehydration: (___ min each) at ___ °C   %    
    %    
    %    
    %    
    %    
    %    
 Absolute ethanol 100% (___ X ___ min/each) at ___ °C 1.    
  2.    
  3.    
Transition: Abs ethanol : Propylene oxide ___ min at ___ °C    
 Propylene oxide 100% (___ X ___ min/each) at ___ °C 1.    
  2.    
  3.    
Infiltration: 33% Spurr's resin (___ hr) at ___ °C    
  67% Spurr's resin (___ hr) at ___ °C    
 100% Spurr's resin (___ X ___ hr/each) at ___ °C 1.    
  2.    
Embed: in new Spurr's resin    
Polymerize: for ___ hr at ___ °C