Lecture: 8:30 - 9:20 am Monday Wednesday Friday
Laboratory: To Be Arranged

References:

• JJ Bozzola & LD Russell. 1999. Electron Microscopy: Principles and Techniques for Biologists, 2^nd ed. ISBN 0-7637-0192-0 (Hardback)
• DB Williams & CB Carter. 1996. Transmission Electron Microscopy: A Textbook for Material Scientists. ISBN 0-306-45247-2 (Hardback) 0-306-45324-X (Paperback)

DATE	LECTURE	LABORATORY	READINGS (Bozzola & Russell, 1999)
Aug 26	Introduction to TEM, biological specimen preparation	Fixation of tissue, laboratory procedures	2-14 [historical perspective]; 616-640 [safety]
Aug 28	Specimen preparation (fixation and buffers)	Dehydrate and embed tissue	16-47 [biological specimen preparation]
Aug 30	Specimen preparation (dehydration and embedding)
Sep 2	Ultramicrotomy, ultramicrotome design, knives	Introduction to Zeiss 10 starts1	72-92; 97-109 [ultramicrotomy]
Sep 4	Intro to TEM instrument, instrument design	Make glass knives and cut thick sections	163-180 [TEM instrument]
Sep 6	vacuum pumps, e- sources	Cut ultrathin sections	180-188 [vacuum pumps]
Sep 9	Labor Day (no class)	*	64-107 [sectioning]; handout on MT-2 ultramicrotome
Sep 11	e- behavior	*	148-162 [light, electrons & lenses]
Sep 13	Electron optics I	Staining	120-130 [staining]; handout on electron optics
Sep 16	Electron optics II	*	188-201 [alignment & operation]
Sep 18	Electron optics III	*
Sep 20	EM photography		240-261 [photography]
Sep 23	Support films, shadow casting, negative staining	Support films, shadow cast, negative stain2	130-147 [negative staining, shadow casting]
Sep 25	Digital imaging, image processing, feature analysis	Digital imaging & feature analysis	414-441 [digital imaging and image processing]
Sep 27	Feature analysis, discuss student prospectus	Prospectus of individual projects due
Sep 30	Specimen-beam interactions, scanning beam technology	Multiple signal acquisition, JEOL 2000	202-231 [scanning]
Oct 2	X-ray spectroscopy	X-ray spectroscopy, JEOL 20002	368-385 [x-ray spectroscopy]
Oct 4	Intermediate and High Voltage EM		396-405 [IVEM and HVEM]
Oct 7	Freezing technology, freeze-substitution	Rapid-freezing, start freeze-substitution3**	Handout: Gilkey and Staehelin, 1986
Oct 9	Freeze-substitution, freeze-drying, freeze-fracture	Freeze fracture3	342-366 [freeze-fracture]
Oct 11	Dallas football weekend
Oct 14	E.M. histochemistry I	Acid phosphatase localization3	282-291 [enzyme cytochemistry]
Oct 16	E.M. histochemistry II	*	292-308 [autoradiography]; 310-318 [other]
Oct 18	E.M. histochemistry III		406-413 [tracers]
Oct 21	Surface labeling & Immunological EM I	Colloidal gold2	262-280 [immunocytochemistry]
Oct 23	Immunological EM II	Cryoultramicrotomy	Handout on immunocytochemistry
Oct 25	Cryoultramicrotomy		109-118
Oct 28	Stereology & morphometry I-sampling	Quantitative cytology2	320-340 [quantitative electron microscopy]
Oct 30	Stereology & morphometry II-serial reconstruction	LM/EM correlation, 3-D reconstruct2	Handout on stereology
Nov 1	Three-dimensional reconstruction	Progress reports due	Handout on 3-D reconstruction
Nov 4	Electron diffraction	Electron diffraction	386-395 [electron diffraction]
Nov 6	Interpretation of electron micrographs	Micrographs & artifacts	442-475 [micrograph interpretation]
Nov 8	Survey of biological ultrastructure		476-614 [atlas of ultrastructure images]
TBA	Preparing illustrations and posters	Preparing posters	Handouts on posters
Dec 13	Presentation of student research posters	Hand-in EMs of standard materials

FINAL EXAMINATION: Wednesday, December 18, 2002, 10:30 am-12:30 PM

*Open laboratory periods may be used for preparation of specimens, ultramicrotomy, microscope use and photography. Instructors will be present during laboratory hours
**Checkout on the Zeiss 10a begins
¹Microscope use outside of regularly scheduled lab periods begins
²Group experiments (1 day project); these are due 1 week after the experiment
³Group experiments (>1 day project); these are due 2 weeks after the experiment OU Administrative pages:

• Fall 2002 Academic Calendar
• Finals Schedule and Rules
• OU Course Policies - this page covers equal opportunity, student code, academic misconduct, attendance policy, final exam policy, reasonable accommodation, sexual harassment, studnet discrimination, and procedures for the release of information about students. These are incorporated by reference into this syllabus and are available online at http://www.ou.edu/bulletins/01catalog/HTML/policies.htm