The staff of the PPCC offers the following services to researchers:
Equipment Use & Training
Protein Expression & Purification
For proteins with established (e.g. published) methods of expression and purification, the core can produce desired quantities of pure protein for a full-service fee (Option A). Alternatively, and for a lower fee, researchers may opt to be involved hands-on with protein expression and purification, with supervision and consultation with the PPCC (Option B).
Instruments are available in the PPCC to characterize proteins and other macromolecules. Interactions between compounds can be detected and analyzed using Isothermal Titration Calorimetry (MicroCal PEAQ-ITC, Malvern) and Biolayer Interferometry (ForteBio Octet Red96). FTIR is available for determination and analysis of secondary structure composition (Tensor II, Bruker). Dynamic Light scattering using the pUNk (Unchained Labs) can be used to assess a protein’s hydrodynamic size, size distribution, and aggregate population.
De Novo Protein Expression & Purification (Developmental Projects)
For proteins with undetermined or partially determined methods of expression and purification, the PPCC can independently develop and execute methods to express and purify the relevant protein for a full-service fee (Option A). Alternatively, the core can collaborate with members of the client lab in developing and enacting a strategy to produce the desired protein (Option B).
We currently do not offer molecular biology services, such as custom cloning. However, once a plasmid is available, transformation into bacteria and protein expression can be done by/in the PPCC.
For researchers in need of custom cloning we suggest using the services of a commercial company, such as Genscript or Invitrogen (ThermoFisher). Please note that we do not endorse a specific company.
There are several websites available for help with cloning strategies and vector selection, among them: