Integrative Immunology Center

Jonathan Savitz, Ph.D. Principal Investigator, Laureate Institute for Brain Research and Associate Professor, University of Tulsa

Projects:

NIMH R01MH123652: Neural response to inflammatory challenge in major depressive disorder. The main goals of this project are aimed at 1) elucidating the neural circuitry underlying a maladaptive homeostatic response to inflammation in MDD by using a placebo-controlled transient inflammatory response and 2) identifying brain targets relevant to inflammation-induced depression.

NIGMS P20GM131212: The Center for Neuroscience-based Mental Health Assessment and Prediction (NeuroMAP): Research Core. The goal of the NeuroMAP is to establish  a  program  to  train  junior  researchers  with  a  pragmatic  focus  to  use  neuroscience  to  improve  mental  health. The components of the Research Core are organized to examine specific units of analyses and create an integrated infrastructure: (A) Symptoms & Behavior, (B) Circuits & Physiology, (C) Cells & Molecules, (D) Intervention, (E) Biostatistics, and (F) IT & Database.

Timothy Meier, Ph.D Assistant Professor of Neurosurgery, Department of Neurosurgery

Project:

NIMH - 1R21NS099789: Inflammation and kynurenine metabolites in the acute sequelae of concussion

Jennifer Hays-Grudo, Ph.D. Professor, Department of Psychiatry and Behavioral Sciences. Center for Integrative Research on Childhood Adversity, National Center for Wellness and Recovery

Projects:

NIMH U01DA055349: 10/24 Healthy Brain and Child Development National Consortium. The main goals of this project are to prospectively examine human brain, cognitive, behavioral, social and emotional development of children and to determine the impact of maternal pre- and postnatal substance use on short- and long-term development of children.

NIGMS P20GM109097: Center for Integrative Research on Childhood Adversity - Biomarker Core. The major goals of this project are to build Oklahoma State University-Tulsa’s (OSU-Tulsa) and OU-Tulsa's research environment focused on discovering the sources of health inequities among children and to translate scientific results into solutions that eliminate children’s health inequities. The Biomarker Core is aimed at providing support for biomarker analyses for the center investigators.

Amanda Sheffield Morris, Ph.D. Regents' Professor and George Kaiser Family Foundation Endowed Chair in Child Development, Department of Psychiatry and Behavioral Sciences, Center for Integrative Research on Childhood Adversity

Projects:

NIMH U01DA055349: 10/24 Healthy Brain and Child Development National Consortium. The main goals of this project are to prospectively examine human brain, cognitive, behavioral, social and emotional development of children and to determine the impact of maternal pre- and postnatal substance use on short- and long-term development of childre

NIGMS P20GM1090907: The Dyadic Inter-Brain Signaling (DIBS) Project: Modeling Parent-Child Inter-Brain Regulation in the Prediction of Adolescent Depressive Symptoms - Center for Integrated Research on Childhood Adversity

Julie Croff, PhD, Professor, Executive Director - Population and Clinical Research, National Center for Wellness and Recovery

Projects:

NIH - P20GM109097: Examining Epidemiology of Folate Status Attributable to Adolescent Alcohol Use - Center for Integrated Research on Childhood Adversity

NIMH U01DA055349: 10/24 Healthy Brain and Child Development National Consortium. The main goals of this project are to prospectively examine human brain, cognitive, behavioral, social and emotional development of children and to determine the impact of maternal pre- and postnatal substance use on short- and long-term development of children.

Bart Ford, PhD. Assistant Professor, Department of  Pharmacology and Physiology

Project: 

Brain & Behavior Research Foundation: Immune Predictors of SARS-CoV-2 Vaccine Response in Major Depressive Disorder. The main goal of this project is to examine the immune predictors of the adaptive immune response to SARS-CoV-2 vaccination in a sample of individuals with mood and anxiety disorders.

Misty Hawkins, PhD,  Assistant Professor, Department of Psychology, College of Arts and Sciences

Projects:

NIH - P20GM109097: Neurotrophic Indicators of Cognition, Executive Skills, Plasticity, and Adverse Childhood Experiences Study (NICE SPACES). Major goals are to 1) Identify whether brain markers of neural health (e.g., neurotrophins) are related to adverse childhood experiences and/or neurocognitive performance, and 2) Test whether neuronal or glial neurotrophins predict or change in response to weight loss.

David Bard, Ph.D. Director, Biomedical and Behavioral Methodology Core (BBMC) , Associate Professor, Department of Pediatrics, OU College of College of Medicine

Project: 

HRSA/OSDH UH4MC307450100: Maternal, Infant, and Early Childhood Home Visiting Program: A Collaborative Learning Innovation for Addressing Engagement/ Retention of Home Visiting

Karina Shreffler, Ph.D. Professor, College of Nursing, University of Oklahoma Health Science Center

Project: 

NIGMS P20GM109097: Holistic Assessment of Tulsa Children's Health (HATCH) - Center for Integrated Research on Childhood Adversity

Marianna Wetherill, Ph.D., M.P.H., RDN/LD, Associate Professor, Department of Health Promotions Sciences, Hudson College of Public Health and Department of Family and Community Medicine, School of Community Medicine

Project: 

NIDDK R01DK127464: Nutrition to Optimize, Understand, and Restore Insulin Sensitivity in HIV for Oklahoma. The major goals of this project are to identify how food insecurity contributes to insulin resistance in HIV and to evaluate a “food as medicine” intervention designed to lower insulin resistance for people living with HIV.

Brett McKinney, Ph.D Professor of Computer Science, William K. Warren, Jr. Endowed Chair in Bioinformatics, College of Engineering and Natural Sciences

Project:

NIGMS P20GM131212: Neuroscience-Based Mental Health Assessment and Prediction (NeuroMAP)- Center of Biomedical Research Excellence - Research Core. The goal of the NeuroMAP is to establish  a  program  to  train  junior  researchers  with  a  pragmatic  focus  to  use  neuroscience  to  improve  mental  health. The components of the Research Core are organized to examine specific units of analyses and create an integrated infrastructure: (A) Symptoms & Behavior, (B) Circuits & Physiology, (C) Cells & Molecules, (D) Intervention, (E) Biostatistics, and (F) IT & Database.

1.      Tsuchiyagaito A, Smith JL, El-Sabbagh N, Zotev V, Misaki M, Al Zoubi O, Kent Teague T, Paulus MP, Bodurka J, Savitz J. Real-time fMRI neurofeedback amygdala training may influence kynurenine pathway metabolism in major depressive disorder. Neuroimage Clin. 2021; 29:102559.  PMID: 33516062

2.      Blair AL, Chiaf AL, Crockett EK, Teague TK, Croff JM. Validation of hair ethyl glucuronide using transdermal monitoring and self-reported alcohol use in women of childbearing potential. Neuropsychopharmacol Rep. 2021 Jun;41(2):144-151. PMID: 33650766

3.      Meier, T. B., España, L. E., Nitta, M. E., Teague, T. K., Brett, B. L., Nelson, L. D., McCrea, M. A., Savitz, J. Positive association between serum quinolinic acid and functional connectivity following concussion. Brain, Behavior, and Immunity. Jan 2021, 91:531-540. PMID: 33176183

4.      Zheng H, Ford BN, Bergamino M, Kuplicki R; Tulsa 1000 Investigators, Hunt PW, Bodurka J, Teague TK, Irwin MR, Yolken RH, Paulus MP, Savitz J. A Hidden Menace? Cytomegalovirus Infection is Associated with Reduced Cortical Gray Matter Volume in Major Depressive Disorder. Molecular Psychiatry. Nov 2020, PMID: 33223520

5.      Savitz, J, Ford, BN, Yeh, HW, Akeman, E, Cosgrove, K, Clausen, A, Martell, C, Kirlic, N, Santiago, J, Teague, TK, Irwin, MR, Paulus, MP, Aupperle, RL. Behavioral Activation Therapy for Depression Reduces the Concentration of Circulating Quinolinic Acid. Psychological Medicine. Nov 2020, PMID: 33234171

6.      Brett, B, Savitz, J, Nitta, M, España, L, Teague, TK, Nelson, L, McCrea, M, Meier, TB.. Systemic inflammation moderates the association of prior concussion with hippocampal volume and episodic memory in high school and collegiate athletes. Brain, Behavior, and Immunity, Oct 2020, 89, 380-388. PMID: 32717401.

7.      Meier, TB, Nitta, ME, Teague, TK, Nelson, LD, McCrea, MC, Savitz, J. Prospective study of the effects of sport-related concussion on serum kynurenine pathway metabolites. Brain, Behavior, and Immunity, Jul 2020, 87, 715-724. PMID: 32147388

8.      Ford BN, Teague TK, Bayouth M, Yolken RH, Bodurka J, Irwin MR, Paulus MP, Savitz J. Diagnosis-independent loss of T-cell costimulatory molecules in individuals with cytomegalovirus infection. Brain, Behavior, and Immunity, Jul 2020, 87:795-803. PMID: 32209361

9.      Meier, TB, Huber, DL, Bohorquez-Montoya, L, Morgan, NE, Savitz, J, Teague, TK, Bazarian, JJ, Hayes, RL, Nelson, LD, McCrea, MA. A prospective study of acute blood-based biomarkers for sport-related concussion. Annals of Neurology, Jun 2020, 87(6), 907-920. PMID: 32215965.

10.  Burrows, K, Stewart, JL, Antonacci, C, Kuplicki, R, Thompson, K, Taylor, A, Teague, TK, Paulus, MP. Association of poorer dietary quality and higher dietary inflammation with greater symptom severity in depressed individuals with appetite loss. Journal of Affective Disorders, Feb 2020, 263, 99-106. PMID: 31818803.

11.  Croff, J, Chibing, T, Chiaf, AL, Hartwell, ML, Crockett, EK, Teague, TK. Erythrocyte and Serum Folate Collection Techniques: A Multi-Method Study of Folate Status. Vitamins & Minerals, 2020, 9, 188.

12.  Cosgrove, KT, Burrows, K, Avery, JA, Kerr, KL, DeVille, DC, Aupperle, RL, Teague, TK, Drevets, WC, Simmons, WK. Appetite change profiles in depression exhibit differential relationships between systemic inflammation and activity in reward and interoceptive neurocircuitry. Brain, Behavior, and Immunity.  2020 Jan. 83, 163-171. PMID: 31604141

13.  Croff, JM, Chiaf, AL, Hartwell, ML, Crockett, EK, Tan, C, Teague, TK. Electronic Nicotine Delivery Systems and Serum Folate: A Case Study. Tobacco Use Insights. Nov 2019. 12, 1-4. PMID: 31798305

14.  Nitta, M., Savitz, J., Nelson, L., Teague, T. K., Hoelzle, J., McCrea, M., Meier, T. Acute elevation of serum inflammatory markers predicts symptom recovery after concussion. Neurology. 2019 Jul 30. 93 (5), e497-e507 PMID: 31270219. 

15.  Ford BN, Yolken RH, Aupperle RL, Teague TK, Irwin MR, Paulus MP, Savitz J. Association of Early-Life Stress With Cytomegalovirus Infection in Adults With Major Depressive Disorder. JAMA Psychiatry. 2019 Mar 6. PMID: 30840037

16.  Ford, BN, Yolken, RH, Dickerson, FB, Teague, TK, Irwin, MR, Paulus, MP, Savitz, J. Reduced Immunity to Measles in Adults with Major Depressive Disorder. Psychological Medicine. Jan 2019. 49 (2), 243-249. PMID: 29552990. 

17.  Le, TT, Savitz, J, Suzuki, H, Misaki, M, Teague, TK, White, B, Marino, JH, Wiley, G, Gaffney, P, Drevets, W, McKinney, B, Bodurka. Identification and replication of RNA-Seq gene network modules associated with depression severity. Translational Psychiatry. Sep 5, 2018 8(1), 180. PMID: 30185774

18.  Simmons, K, Burrows, K, Avery, J, Kerr, K, Taylor, A, Bodurka, J, Potter, W, Teague, TK, Drevets, W.  Appetite Changes Reveal Depression Subgroups with Distinct Endocrine, Metabolic, and Immune States. Molecular Psychiatry. 2018 Jun 13. PMID: 29899546

19.  Savitz J, Teague TK, Misaki M, Macaluso M, Wurfel B., Meyer M., Drevets D, Yates W, Gleason OC, Drevets W, Preskorn S. Treatment of Bipolar Depression with Minocycline and/or Aspirin: An adaptive, 2 X 2 double-blind, randomized, placebo-controlled, phase IIA clinical trial. Transl Psychiatry. 2018 Jan 24;8(1):27. PMID: 29362444

20.  Meier TB, Drevets WC, Teague TK, Wurfel BE, Mueller SC, Bodurka J, Dantzer R, Savitz J. Kynurenic acid is reduced in females and oral contraceptive users: Implications for depression. Brain, Behavior, and immunity. 2018 Jan 67:59-64. PMID: 28867283

21.  Suzuki H, Savitz, J, Teague TK, Gandhapudi SK, Tan, C, Misaki M, McKinney BA, Irwin, MR, Drevets, WC, Bodurka, J. Altered Populations of Natural Killer Cells, Cytotoxic T Lymphocytes, and Regulatory T Cells in Major Depressive Disorder: Association with Sleep Disturbance. Brain Behav Immun. 2017 Nov 66:193-200. PMID: 28645775 

22.  Cho HJ, Savitz J, Dantzer R, Teague TK, Drevets WC, Irwin MR. Sleep disturbance and kynurenine metabolism in depression. J Psychosom Res. 2017 Aug;99:1-7. PMID: 28712413.

23.  Wurfel BE, Drevets WC, Bliss SA, McMillin JR, Suzuki H, Ford BN, Morris HM, Teague TK, Dantzer R, Savitz JB. Serum kynurenic acid is reduced in affective psychosis. Transl Psychiatry. 2017 May 2;7(5):e1115. PMID: 28463241.

24.  Meier TB, Lancaster MA, Mayer AR, Teague TK, Savitz J. Abnormalities in Functional Connectivity in Collegiate Football Athletes with and without a Concussion History: Implications and Role of Neuroactive Kynurenine Pathway Metabolites. J Neurotrauma. 2017 Feb 15;34(4):824-837. PMID: 27618518.

25.  Young KD, Drevets WC, Dantzer R, Teague TK, Bodurka J, Savitz J. Kynurenine pathway metabolites are associated with hippocampal activity during autobiographical memory recall in patients with depression. Brain Behav Immun. 2016 Aug;56:335-42. PMID: 27091600

26.  Meier TB, Savitz J, Singh R, Teague TK, Bellgowan PS. Smaller Dentate Gyrus and CA2 and CA3 Volumes Are Associated with Kynurenine Metabolites in Collegiate Football Athletes. J Neurotrauma. 2016 Jul 15;33(14):1349-57. PMID: 26493952

27.  Singh R, Savitz J, Teague TK, Polanski DW, Mayer AR, Bellgowan PS, Meier TB. Mood symptoms correlate with kynurenine pathway metabolites following sports-related concussion. J Neurol Neurosurg Psychiatry. 2016 Jun;87(6):670-5. PMID: 26269650.

28.  Meier TB, Drevets WC, Wurfel BE, Ford BN, Morris HM, Victor TA, Bodurka J, Teague TK, Dantzer R, Savitz J. Relationship between neurotoxic kynurenine metabolites and reductions in right medial prefrontal cortical thickness in major depressive disorder. Brain Behav Immun. 2016 Mar;53:39-48. PMID: 26546831

29.  Meier TB, Bergamino M, Bellgowan PS, Teague TK, Ling JM, Jeromin A, Mayer AR. Longitudinal assessment of white matter abnormalities following sports-related concussion. Hum Brain Mapp. 2016 Feb;37(2):833-45. PMID: 26663463.

30.  Savitz J, Dantzer R, Meier TB, Wurfel BE, Victor TA, McIntosh SA, Ford BN, Morris HM, Bodurka J, Teague TK, Drevets WC. Activation of the kynurenine pathway is associated with striatal volume in major depressive disorder. Psychoneuroendocrinology. 2015 Dec;62:54-

31.  Savitz J, Drevets WC, Wurfel BE, Ford BN, Bellgowan PS, Victor TA, Bodurka J, Teague TK, Dantzer R. Reduction of kynurenic acid to quinolinic acid ratio in both the depressed and remitted phases of major depressive disorder. Brain Behav Immun. 2015 May;46:55-9. PMID: 25686798

32.  Gandhapudi SK, Tan C, Marino JH, Taylor AA, Pack CC, Gaikwad J, Van De Wiele CJ, Wren JD, Teague TK. IL-18 acts in synergy with IL-7 to promote ex vivo expansion of T lymphoid progenitor cells. J Immunol. 2015 Apr 15;194(8):3820-8. PMID: 25780034

33.  Savitz J, Dantzer R, Wurfel BE, Victor TA, Ford BN, Bodurka J, Bellgowan PS, Teague TK, Drevets WC. Neuroprotective kynurenine metabolite indices are abnormally reduced and positively associated with hippocampal and amygdalar volume in bipolar disorder. Psychoneuroendocrinology. 2015 Feb;52:200-11. PMID: 25486577

34.  Savitz J, Drevets WC, Smith CM, Victor TA, Wurfel BE, Bellgowan PS, Bodurka J, Teague TK, Dantzer R. Putative neuroprotective and neurotoxic kynurenine pathway metabolites are associated with hippocampal and amygdalar volumes in subjects with major depressive disorder.Neuropsychopharmacology. 2015 Jan;40(2):463-71. PMID: 25074636

35.  Duffy FD, Miller-Cribbs JE, Clancy GP, Van De Wiele CJ, Teague TK, Crow S, Kollaja EA, Fox MD. Changing the culture of a medical school by orienting students and faculty toward community medicine. Acad Med. 2014 Dec;89(12):1630-5. PMID: 25162616.

36.  Singh R, Meier TB, Kuplicki R, Savitz J, Mukai I, Cavanagh L, Allen T, Teague TK, Nerio C, Polanski D, Bellgowan PS. Relationship of collegiate football experience and concussion with hippocampal volume and cognitive outcomes. JAMA. 2014 May 14;311(18):1883-8. PMID: 24825643.

37.  Savitz J, Frank MB, Victor T, Bebak M, Marino JH, Bellgowan PS, McKinney BA, Bodurka J, Kent Teague T, Drevets WC. Inflammation and neurological disease-related genes are differentially expressed in depressed patients with mood disorders and correlate with morphometric and functional imaging abnormalities. Brain Behav Immun. 2013 Jul;31:161-71. PMID: 23064081

38.  Savitz J, Preskorn S, Teague TK, Drevets D, Yates W, Drevets W. Minocycline and aspirin in the treatment of bipolar depression: a protocol for a proof-of-concept, randomised, double-blind, placebo-controlled, 2x2 clinical trial. BMJ Open. 2012 Feb 22;2(1):e000643. PMID: 22357572

39.  Tan C, Taylor AA, Coburn MZ, Marino JH, Van De Wiele CJ, Teague TK. Ten-color flow cytometry reveals distinct patterns of expression of CD124 and CD126 by developing thymocytes. BMC Immunol. 2011 Jun 20;12:36. PMID:

40.  Li X, Naylor MF, Le H, Nordquist RE, Teague TK, Howard CA, Murray C, Chen WR. Clinical effects of in situ photoimmunotherapy on late-stage melanoma patients: a preliminary study. Cancer Biol Ther. 2010 Dec 1;10(11):1081-7.  PMID: 20890121

41.  Teague TK, Tan C, Marino JH, Davis BK, Taylor AA, Huey RW, Van De Wiele CJ. CD28 expression redefines thymocyte development during the pre-T to DP transition. Int Immunol. 2010 May;22(5):387-97. PMID: 20203098.

42.  Marino JH, Tan C, Taylor AA, Bentley C, Van De Wiele CJ, Ranne R, Paliotta M, Broughan TA, Teague TK. Differential IL-7 responses in developing human thymocytes. Hum Immunol. 2010 Apr;71(4):329-33. PMID: 20074604

43.  Marino JH, Tan C, Davis B, Han ES, Hickey M, Naukam R, Taylor A, Miller KS, Van De Wiele CJ, Teague TK. Disruption of thymopoiesis in ST6Gal I-deficient mice. Glycobiology. 2008 Sep;18(9):719-26. PMID: 18535087

44.  Broughan TA, Naukam R, Tan C, Van De Wiele CJ, Refai H, Teague TK. Effects of hepatic zonal oxygen levels on hepatocyte stress responses. J Surg Res. 2008 Mar;145(1):150-60. PMID: 18164035.

45.  Van de Wiele CJ, Marino JH, Tan C, Kneale HA, Weber J, Morelli JN, Davis BK, Taylor AA, Teague TK. Impaired thymopoiesis in interleukin-7 receptor transgenic mice is not corrected by Bcl-2. Cell Immunol. 2007 Nov-Dec;250(1-2):31-9. PMID: 18321477

46.  Naylor MF, Chen WR, Teague TK, Perry LA, Nordquist RE. In situ photoimmunotherapy: a tumour-directed treatment for melanoma. Br J Dermatol. 2006 Dec;155(6):1287-92. PMID: 17107404.

47.  Marino JH, Van De Wiele CJ, Everhart JM, Masengale R, Naukam RJ, Schniederjan MJ, Vo S, Teague TK. Attenuation of cytokine responsiveness during T cell development and differentiation. J Interferon Cytokine Res. 2006 Oct;26(10):748-59. PMID: 17032169.

48.  Rao RS, Howard CA, Teague TK. Pulmonary endothelial permeability is increased by fluid from packed red blood cell units but not by fluid from clinically-available washed units. J Trauma. 2006 Apr;60(4):851-8. PMID: 16612308.

49.  Van De Wiele CJ, Marino JH, Murray BW, Vo SS, Whetsell ME, Teague TK. Thymocytes between the beta-selection and positive selection checkpoints are nonresponsive to IL-7 as assessed by STAT-5 phosphorylation. J Immunol. 2004 Apr 1;172(7):4235-44. PMID: 15034036.

50.  Van De Wiele CJ, Marino JH, Whetsell ME, Vo SS, Masengale RM, Teague TK. Loss of interferon-induced Stat1 phosphorylation in activated T cells. J Interferon Cytokine Res. 2004 Mar;24(3):169-78. PMID: 15035850.

51.   Naylor MF, Crowson N, Kuwahara R, Teague K, Garcia C, Mackinnis C, Haque R, Odom C, Jankey C, Cornelison RL. Treatment of lentigo maligna with topical imiquimod. Br J Dermatol. 2003 Nov;149 Suppl 66:66-70. PMID: 14616356.

Certified flow cytometrists: Ashlee Rempel, B.S., M.B.A. and Chibing Tan, Ph.D.

For instrument availability or laboratory protocol information contact:
Ashlee Rempel, B.S., M.B.A.
Phone: 918-660-3924
Fax: 918-660-3928
Email: ashlee-rempel@ouhsc.edu

AutoMACS cell sorter (Miltenyi Biotec) This instrument is used for magnetic bead cell enrichment and is used to quickly enrich cells from very large starting populations.

For laboratory protocol information contact:
Julie Marino, PhD
Phone: 918-660-3934
Fax: 918-660-3928
Email: julie-marino@ouhsc.edu

MESO QUICKPLEX SQ 120 (Meso Scale Discovery) is an instrument  that uses technology similar to plate-bound ELISAs but is specifically designed for high-sensitivity multiplexed assays.  This instrument allows for highly sensitive analysis of cytokines and other biomarkers using small sample sizes and low inter-assay variability.

SmartCycler Real-Time PCR machine (Cepheid) This instrument has 3 separate units and can analyze 48 samples independently using up to four fluorochromes simultaneously.  

ImageQuant LAS4000 (GE) This hardware/software package allows for image capture and analysis of Western blots, DNA and PCR gels, culture dishes and microscope fields.

For laboratory protocol information contact:
Brenda Davis, BS
Phone: 918-660-3910
Fax: 918-660-3928
Email: brenda-davis@ouhsc.edu

1R01DK127464-01 (Wetherill) 09/17/2020 – 08/31/2025

NIH/NIDDK

Nutrition to Optimize, Understand, and Restore Insulin Sensitivity in HIV for Oklahoma (NOURISH-OK): The goal of this project is to determine how food insecurity and chronic inflammation relate to insulin resistance via diet, other health behaviors, and the microbiome. The IIC laboratories will be providing biomarker assessments for this study. 

P20GM131212-01 (Paulus) 09/15/2017 – 06/30/2022

NIH/NIGMS

The Center for Neuroscience-based Mental Health Assessment and Prediction (NeuroMAP) – The IIC serves as a core biomarker laboratory for the research projects and interventions for this multicenter project.

P20GM109097 sub #6025 (Teague) 07/01/2016 – 03/31/2022

NIH/NIGMS

Center for Integrative Research on Childhood Adversity (previously the Children's Health Equity Solutions Center) –The IIC serves as the biomarker core laboratories for the research projects and interventions for this multicenter project.